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Micro RNA's
The CombiMatrix MicroRNA product suite is built on the CustomArray™ 4X2K format. The catalog Species Specific MicroRNA arrays consist of Human, Mouse, Mouse/Rat, Rat, C. elegans, Drosophila, Arabidopsis and Maize microarrays built with probes from the Sanger Database (version 9.0).
Species Specific MicroRNAs are listed below. To see the probe list of any of the MicroRNAs, click on the link in the table.
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Human |
Homo sapiens |
500 |
32 |
1000071-4985 |
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Rat |
Rattus norvegicus |
439 |
33 |
1000071-4990 |
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Mouse |
Mus musculus |
439 |
33 |
1000071-4989 |
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C.Elegans |
Caenorhabditis elegans/
Caenorhabditis briggsae |
225 |
33 |
1000071-4984 |
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Fruit fly |
Drosphila Melanogaster/
Drosphila psuedoobscrua |
109 |
32 |
1000071-4987 |
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Arabidopsis |
Arabidopsis thaliana |
294 |
32 |
1000071-4983 |
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Maize |
Zea mays |
294 |
29 |
1000071-4986 |
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Designs are based on unique microRNA species from current release of Sanger database (Version 9.0 is at http://microrna.sanger.ac.uk/sequences/) |
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Hairpin sequences are excluded from the design process |
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Probes are anti-sense to mature microRNA |
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For each probe, a double mismatch control is included to ensure specificity of detection. These ‘2mut’ controls are designed to maximally de-stabilize probe-target hybridization, thus, mutations are introduced in the two most stable triplets. |
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Non-microRNA controls include positive controls corresponding to small RNA such as tRNA and U6. The small RNA co-isolate with microRNA if the target preparation protocol is based on size fractionation. |
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The negative controls contain anti-sense probes designed for abundant mRNA (such as Actb (actin), HSPA4, PGK1). If target samples have some RNA degradation, the partially digested fragments of the abundant mRNA will co-isolate with microRNA during size fractionation, and the corresponding probes will produce high intensity signals. If target samples are of good quality, the negative control signals will stay low. |
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MicroRNA Microarrays are compatible with common reagents and scanners |
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All probes on every microRNA are 100% quality tested |
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30 day turn-around time |
Users can select the subset of organisms from the Sanger 9.0 database for the Content Choice MicroRNA offered by CombiMatrix. Users define the content as well as the controls on the MicroRNA.
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for more information